Sema4C, a Transmembrane Semaphorin, Interacts with a Post-synaptic Density Protein, PSD-95
Copyright policy )Sema4C, a Transmembrane Semaphorin, Interacts with a Post-synaptic Density Protein, PSD-95
Semaphorins are known to act as chemorepulsive molecules that guide axons during neural development. Sema4C, a group 4 semaphorin, is a transmembrane semaphorin of unknown function. The cytoplasmic domain of Sema4C contains a proline-rich region that may interact with some signaling proteins. In this study, we demonstrate that Sema4C is enriched in the adult mouse brain and associated with PSD-95 isoforms containing PDZ (PSD-95/DLG/ZO-1) domains, such as PSD-95/SAP90, PSD-93/chapsin110, and SAP97/DLG-1, which are concentrated in the post-synaptic density of the brain. In the neocortex, S4C is enriched in the synaptic vesicle fraction and Triton X-100 insoluble post-synaptic density fraction. Immunostaining for Sema4C overlaps that for PSD-95 in superficial layers I-IV of the neocortex. In neocortical culture, S4C is colocalized with PSD-95 in neurons, with a dot-like pattern along the neurites. Sema4C thus may function in the cortical neurons as a bi-directional transmembrane ligand through interacting with PSD-95.
- Osaka University Japan
Cerebral Cortex, Binding Sites, Base Sequence, Intracellular Signaling Peptides and Proteins, Gene Expression Regulation, Developmental, Membrane Proteins, Nerve Tissue Proteins, Semaphorins, Rats, Mice, Two-Hybrid System Techniques, Animals, Disks Large Homolog 4 Protein, Guanylate Kinases, DNA Primers, Protein Binding, Subcellular Fractions
Cerebral Cortex, Binding Sites, Base Sequence, Intracellular Signaling Peptides and Proteins, Gene Expression Regulation, Developmental, Membrane Proteins, Nerve Tissue Proteins, Semaphorins, Rats, Mice, Two-Hybrid System Techniques, Animals, Disks Large Homolog 4 Protein, Guanylate Kinases, DNA Primers, Protein Binding, Subcellular Fractions
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