Dense Chromatin Activates Polycomb Repressive Complex 2 to Regulate H3 Lysine 27 Methylation
Copyright policy )Dense Chromatin Activates Polycomb Repressive Complex 2 to Regulate H3 Lysine 27 Methylation
Maintaining Repression The Polycomb Repressive Complex 2 (PRC2) plays a critical role in gene silencing in metazoans, methylating histone H3 on lysine 27 (H3K27) to generate a repressive chromatin mark. The catalytic subunit E(z)/Ezh2 requires the presence of two other subunits—ESC/EED and Su(z)12—for enzyme activity. Yuan et al. (p. 971 ; see the Perspective by Pirrotta ) show that both a fragment of the histone H3 N-terminal tail, and histone H1 stimulated PRC2 enzyme activity on poor, low-density chromatin substrates, indicating that that PRC2 is regulated by the density and compaction states of chromatin. The histone H3 fragment binds to the Su(z)12 subunit of PRC2 to stimulate E(z)/Ezh2. Local chromatin compaction preceded establishment of histone H3K27 methylation indicating how PRC2 might maintain the repressed state.
- PEKING UNION MEDICAL COLLEGE China (People's Republic of)
- University of Southern California United States
- University of California System United States
- National Institute of Biological Sciences, Beijing China (People's Republic of)
- University of Minnesota System United States
CD4-Positive T-Lymphocytes, Chromatin Immunoprecipitation, Lysine, Molecular Sequence Data, Polycomb Repressive Complex 2, Histone-Lysine N-Methyltransferase, Methylation, Peptide Fragments, Nucleosomes, Histones, Mice, Drosophila melanogaster, Cytochrome P-450 Enzyme System, Mutagenesis, Animals, Drosophila Proteins, Humans, Amino Acid Sequence, Gene Silencing, Embryonic Stem Cells
CD4-Positive T-Lymphocytes, Chromatin Immunoprecipitation, Lysine, Molecular Sequence Data, Polycomb Repressive Complex 2, Histone-Lysine N-Methyltransferase, Methylation, Peptide Fragments, Nucleosomes, Histones, Mice, Drosophila melanogaster, Cytochrome P-450 Enzyme System, Mutagenesis, Animals, Drosophila Proteins, Humans, Amino Acid Sequence, Gene Silencing, Embryonic Stem Cells
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