
(A) Actin depolymerization was monitored by the decrease in pyrene fluorescence and normalized to the initial fluorescence value in each experiment. (B) Summary of pyrene fluorescence at 600 s. Data represent the mean and SE from three independent experiments. *, P < 0.01 versus nonphosphorylated AQP2 liposomes. (C and D) Pyrene-actin assay with barbed-end blocked actin filaments, TM5b, and AQP2 reconstituted in liposomes. (C) Depolymerization curves normalized to the initial fluorescence value in each experiment. (D) Summary of pyrene fluorescence at 600 s. Data represent the mean and SE from three independent experiments. *, P < 0.01 versus actin + TM5b; **, P < 0.05 versus AQP2 liposome + actin + TM5b.Copyright information:Taken from "Reciprocal interaction with G-actin and tropomyosin is essential for aquaporin-2 trafficking"The Journal of Cell Biology 2008;182(3):587-601.Published online 11 Aug 2008PMCID:PMC2500142.
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