Lentiviral gene transfer of CTLA4 generates B cells with reduced costimulatory properties
Copyright policy )Lentiviral gene transfer of CTLA4 generates B cells with reduced costimulatory properties
Peripheral T-cell (TC) tolerance can be induced by tolerogenic antigen-presenting cell (APC). A prerequisite is the reduction or blockade of B7 of APC. Besides dendritic cell, B cells can be used as APC. Here, we show the generation B cells with reduced B7 expression by lentiviral transduction of endoplasmic reticulum (ER)-directed CTLA4. Vectors coding for the human CTL4-Ig were used for the human B-cell line Raji. Transduction efficiency was over 90% (MOI = 3). For the murine B-cell line A20 and for primary mouse B cells, murine CTLA4 was used. We show that B cells with reduced B7 expression reduce the antigen (Ag) specific TC proliferation in vitro. B cells expressing an ER-directed CTLA4 may reduce Ag-specific immune responses.
- Beuth University of Applied Sciences Germany
- Novartis (Switzerland) Switzerland
- University of Potsdam Germany
- Charité - University Medicine Berlin Germany
Antigen Presentation, B-Lymphocytes, Genetic Vectors, Lentivirus, Gene Transfer Techniques, Antigen-Presenting Cells, Endoplasmic Reticulum, Lymphocyte Activation, Cell Line, Mice, Antigens, CD, B7-1 Antigen, Immune Tolerance, Animals, Humans, CTLA-4 Antigen, Institut für Biochemie und Biologie
Antigen Presentation, B-Lymphocytes, Genetic Vectors, Lentivirus, Gene Transfer Techniques, Antigen-Presenting Cells, Endoplasmic Reticulum, Lymphocyte Activation, Cell Line, Mice, Antigens, CD, B7-1 Antigen, Immune Tolerance, Animals, Humans, CTLA-4 Antigen, Institut für Biochemie und Biologie
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