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Molecular and Cellular Biology
Article . 2003 . Peer-reviewed
License: ASM Journals Non-Commercial TDM
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The Drosophila Selenoprotein BthD Is Required for Survival and Has a Role in Salivary Gland Development

Authors: Kwon, So Yeon; Badenhorst, Paul; Martin-Romero, F. Javier; Carlson, Bradley A.; Paterson, Bruce M.; Gladyshev, Vadim; Jae Lee, Byeong; +1 Authors

The Drosophila Selenoprotein BthD Is Required for Survival and Has a Role in Salivary Gland Development

Abstract

Selenium is implicated in many diseases, including cancer, but its function at the molecular level is poorly understood. BthD is one of three selenoproteins recently identified in Drosophila. To elucidate the function of BthD and the role of selenoproteins in cellular metabolism and health, we analyzed the developmental expression profile of this protein and used inducible RNA interference (RNAi) to ablate function. We find that BthD is dynamically expressed during Drosophila development. bthD mRNA and protein are abundant in the ovaries of female flies and are deposited into the developing oocyte. Maternally contributed protein and RNA persist during early embryonic development but decay by the onset of gastrulation. At later stages of embryogenesis, BthD is expressed highly in the developing salivary gland. We generated transgenic fly lines carrying an inducible gene-silencing construct, in which an inverted bthD genomic-cDNA hybrid is under the control of the Drosophila Gal4 upstream activation sequence system. Duplex RNAi induced from this construct targeted BthD mRNA for destruction and reduced BthD protein levels. We found that loss of BthD compromised salivary gland morphogenesis and reduced animal viability.

Country
United States
Keywords

570, DNA, Complementary, Base Sequence, Biophysics, 610, Gene Expression Regulation, Developmental, Proteins, Biochemistry, Salivary Glands, Animals, Genetically Modified, Phenotype, and Structural Biology, Animals, Drosophila Proteins, Drosophila, Female, RNA Interference, RNA, Messenger, Selenoproteins, Subcellular Fractions

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    citations
    This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    13
    popularity
    This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
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    influence
    This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
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    impulse
    This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
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citations
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
13
Average
Average
Average
bronze
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