mGluR5 and NMDA Receptors Drive the Experience- and Activity-Dependent NMDA Receptor NR2B to NR2A Subunit Switch
Copyright policy )mGluR5 and NMDA Receptors Drive the Experience- and Activity-Dependent NMDA Receptor NR2B to NR2A Subunit Switch
In cerebral cortex there is a developmental switch from NR2B- to NR2A-containing NMDA receptors (NMDARs) driven by activity and sensory experience. This subunit switch alters NMDAR function, influences synaptic plasticity, and its dysregulation is associated with neurological disorders. However, the mechanisms driving the subunit switch are not known. Here, we show in hippocampal CA1 pyramidal neurons that the NR2B to NR2A switch driven acutely by activity requires activation of NMDARs and mGluR5, involves PLC, Ca(2+) release from IP(3)R-dependent stores, and PKC activity. In mGluR5 knockout mice the developmental NR2B-NR2A switch in CA1 is deficient. Moreover, in visual cortex of mGluR5 knockout mice, the NR2B-NR2A switch evoked in vivo by visual experience is absent. Thus, we establish that mGluR5 and NMDARs are required for the activity-dependent NR2B-NR2A switch and play a critical role in experience-dependent regulation of NMDAR subunit composition in vivo.
- University of Bristol United Kingdom
- National Institute of Health Pakistan
- National Institutes of Health United States
Male, 570, N-Methylaspartate, Pyridines, Neuroscience(all), 610, In Vitro Techniques, Hippocampus, Models, Biological, Mice, Piperidines, Excitatory Amino Acid Agonists, Animals, Enzyme Inhibitors, Estrenes, Mice, Knockout, Pyramidal Cells, Excitatory Postsynaptic Potentials, Adaptation, Physiological, Electric Stimulation, Animals, Newborn, Female, Excitatory Amino Acid Antagonists
Male, 570, N-Methylaspartate, Pyridines, Neuroscience(all), 610, In Vitro Techniques, Hippocampus, Models, Biological, Mice, Piperidines, Excitatory Amino Acid Agonists, Animals, Enzyme Inhibitors, Estrenes, Mice, Knockout, Pyramidal Cells, Excitatory Postsynaptic Potentials, Adaptation, Physiological, Electric Stimulation, Animals, Newborn, Female, Excitatory Amino Acid Antagonists
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