Cloning and Characterization of the Murine Genes for bHLH-ZIP Transcription Factors TFEC and TFEB Reveal a Common Gene Organization for All MiT Subfamily Members
Copyright policy )Cloning and Characterization of the Murine Genes for bHLH-ZIP Transcription Factors TFEC and TFEB Reveal a Common Gene Organization for All MiT Subfamily Members
The microphthalmia-TFE (MiT) subfamily of basic helix-loop-helix leucine zipper (bHLH-ZIP) transcription factors, including TFE3, TFEB, TFEC, and Mitf, has been implicated in the regulation of tissue-specific gene expression in several cell lineages. In this report, we investigate the genomic organization and structural relatedness of MiT transcription factors. We characterized the gene for mTFEC, which covers a region of more than 50 kb and is composed of seven exons. Further, we cloned a cDNA for the murine TFEB homologue and characterized its genomic structure. The eight coding exons of mTFEB are distributed over a 6-kb region. A multiple alignment of amino acid sequences of known MiT subfamily members indicates undescribed, conserved N-terminal regions and common putative phosphorylation sites for TFE3, TFEB, and Mitf. Also, intron-exon borders for characterized MiT genes appear completely conserved. A new family member and closely related putative transcription factor in Caenorhabditis elegans was identified by database searches that show a similar genomic organization within the bHLH-ZIP region and the acidic domain. Evolutionary aspects and implications for structure-function relationships are discussed.
- The Ohio State University United States
- University of Queensland Australia
- University of Queensland Australia
- The Ohio State University at Marion United States
- University of Queensland Australia
570, Leucine Zippers, Base Sequence, Basic Helix-Loop-Helix Leucine Zipper Transcription Factors, Helix-Loop-Helix Motifs, Molecular Sequence Data, Restriction Mapping, Physical Chromosome Mapping, DNA-Binding Proteins, Evolution, Molecular, Mice, 1311 Genetics, Genetics, Animals, Humans, Amino Acid Sequence, Caenorhabditis elegans, Sequence Alignment, Conserved Sequence, Transcription Factors
570, Leucine Zippers, Base Sequence, Basic Helix-Loop-Helix Leucine Zipper Transcription Factors, Helix-Loop-Helix Motifs, Molecular Sequence Data, Restriction Mapping, Physical Chromosome Mapping, DNA-Binding Proteins, Evolution, Molecular, Mice, 1311 Genetics, Genetics, Animals, Humans, Amino Acid Sequence, Caenorhabditis elegans, Sequence Alignment, Conserved Sequence, Transcription Factors
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