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Scission ofCOPIandCOPIIVesicles Is Independent ofGTPHydrolysis

descriptionPublicationkeyboard_double_arrow_right Article 10 Jun 2013 English Publisher:WileyJournal:Traffic, volume 14, pages 922-932 (issn: 1398-9219, eissn: 1600-0854,

Authors: Andrea Hellwig; Rainer D. Beck; Britta Brügger; Frank Adolf; Alexia Herrmann; Felix T. Wieland;

doi: 10.1111/tra.12084

pmid: 23691917

Scission ofCOPIandCOPIIVesicles Is Independent ofGTPHydrolysis

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Abstract

SynopsisThe question as to whether GTP is hydrolyzed for the scission of a coated bud to release a transport vesicle from a donor membrane is pivotal for elucidating the mechanism of the scission step proper. We have revisited the requirement for GTP hydrolysis in the formation of COP vesicles in semi‐intact cells in combination with recombinant COPI and COPII coat proteins and their respective small GTPases (Arf1 and Sar1). Both types of vesicles were efficiently formed and released without the need of GTP hydrolysis or mechanical manipulation.

Related Organizations

Heidelberg University
Germany

Keywords

Cricetinae, Hydrolysis, Mutation, Vesicular Transport Proteins, Animals, Humans, ADP-Ribosylation Factor 1, Guanosine Triphosphate, COP-Coated Vesicles, HeLa Cells

13 Research products, page 1 of 2

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