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Nucleic Acids Research
Article . 1979 . Peer-reviewed
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Nucleic Acids Research
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Nucleic Acids Research
Article . 1979
Data sources: Europe PubMed Central
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Nucleic Acids Research
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Construction and analysis of recombinant DNAs containing a structural gene for rat prolactin

descriptionPublicationkeyboard_double_arrow_right Article 01 Jan 1979Publisher:Oxford University Press (OUP)Journal:Nucleic Acids Research, volume 6, pages 915-930 (issn: 0305-1048, eissn: 1362-4962, Copyright policy )
Authors: Richard A. Maurer; James L. Hartley; John E. Donelson; Earl J. Gubbins;

doi: 10.1093/nar/6.3.915

pmid: 375200

pmc: PMC327742

Construction and analysis of recombinant DNAs containing a structural gene for rat prolactin

Abstract

Poly A-containing RNA enriched in prolactin-coding sequences was isolated from female rate pituitaries after induction with diethylstilbesterol. Double stranded cDNA was synthesized from this RNA and inserted into plasmid pBR322 at the Pst I site via the poly(dG):polyy(dC) tailing method. E. coli was transformed with this DNA and the recombinant plasmid in one of the transformants characterized in detail. About half of its 900 base pair cDNA insert was sequenced. The DNA sequence is consistent with most of the reported amino acid sequence of rat preprolactin. In addition, the recombinant plasmids in two of the other transformants appear to contain growth hormone coding sequences.

Related Organizations
Keywords

Base Sequence, Transcription, Genetic, DNA, Recombinant, Prolactin, Rats, Genes, Pituitary Gland, Protein Biosynthesis, Escherichia coli, Animals, Nucleic Acid Conformation, Female, Amino Acid Sequence, RNA, Messenger, Poly A, Diethylstilbestrol, Plasmids

  • BIP!
    Impact byBIP!
    citations
    This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    		 96
    popularity
    This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
    		 Average
    influence
    This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    		 Top 1%
    impulse
    This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
    		 Top 10%
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citations
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
96
Average
Top 1%
Top 10%
gold