TTC26/DYF13 is an intraflagellar transport protein required for transport of motility-related proteins into flagella
Copyright policy )TTC26/DYF13 is an intraflagellar transport protein required for transport of motility-related proteins into flagella
Cilia/flagella are assembled and maintained by the process of intraflagellar transport (IFT), a highly conserved mechanism involving more than 20 IFT proteins. However, the functions of individual IFT proteins are mostly unclear. To help address this issue, we focused on a putative IFT protein TTC26/DYF13. Using live imaging and biochemical approaches we show that TTC26/DYF13 is an IFT complex B protein in mammalian cells and Chlamydomonas reinhardtii. Knockdown of TTC26/DYF13 in zebrafish embryos or mutation of TTC26/DYF13 in C. reinhardtii, produced short cilia with abnormal motility. Surprisingly, IFT particle assembly and speed were normal in dyf13 mutant flagella, unlike in other IFT complex B mutants. Proteomic and biochemical analyses indicated a particular set of proteins involved in motility was specifically depleted in the dyf13 mutant. These results support the concept that different IFT proteins are responsible for different cargo subsets, providing a possible explanation for the complexity of the IFT machinery.
- University of California, San Francisco United States
- University of Tokyo Japan
- Osaka University Japan
- Max Planck Society Germany
- Texas A&M University United States
570, Biomedical and clinical sciences, Embryo, Nonmammalian, Genotype, QH301-705.5, 1.1 Normal biological development and functioning, Science, Bioinformatics and Computational Biology, Transfection, Cell Line, Mice, Underpinning research, Cell Movement, Animals, Developmental, Cilia, Biology (General), axoneme, Zebrafish, Plant Proteins, Nonmammalian, dynein, Chlamydomonas, Q, Algal Proteins, R, Intracellular Signaling Peptides and Proteins, Health sciences, Gene Expression Regulation, Developmental, Cell Biology, Biological Sciences, Zebrafish Proteins, Biological sciences, Protein Transport, Phenotype, Gene Expression Regulation, Embryo, Flagella, Gene Knockdown Techniques, Mutation, Medicine, Biochemistry and Cell Biology, Generic health relevance, flagella, Carrier Proteins, Chlamydomonas reinhardtii
570, Biomedical and clinical sciences, Embryo, Nonmammalian, Genotype, QH301-705.5, 1.1 Normal biological development and functioning, Science, Bioinformatics and Computational Biology, Transfection, Cell Line, Mice, Underpinning research, Cell Movement, Animals, Developmental, Cilia, Biology (General), axoneme, Zebrafish, Plant Proteins, Nonmammalian, dynein, Chlamydomonas, Q, Algal Proteins, R, Intracellular Signaling Peptides and Proteins, Health sciences, Gene Expression Regulation, Developmental, Cell Biology, Biological Sciences, Zebrafish Proteins, Biological sciences, Protein Transport, Phenotype, Gene Expression Regulation, Embryo, Flagella, Gene Knockdown Techniques, Mutation, Medicine, Biochemistry and Cell Biology, Generic health relevance, flagella, Carrier Proteins, Chlamydomonas reinhardtii
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