CUL3andNRF2Mutations Confer an NRF2 Activation Phenotype in a Sporadic Form of Papillary Renal Cell Carcinoma
Copyright policy )CUL3andNRF2Mutations Confer an NRF2 Activation Phenotype in a Sporadic Form of Papillary Renal Cell Carcinoma
AbstractSustained activation of the stress-regulated transcription factor NRF2 (NFE2L2) is a prominent feature of many types of cancer, implying that mutations driving NRF2 may be important to tumor progression. In hereditary type 2 papillary renal cell carcinoma (PRCC2, also known as hereditary leiomyomatosis and renal cell cancer), NRF2 activation is a direct consequence of the accumulation of intracellular fumarate, a result of fumarate hydratase (FH) inactivation, but it is not clear how NRF2 may be activated in sporadic forms of PRCC2. Here we show that somatic mutations in NRF2, CUL3, and SIRT1 are responsible for driving the NRF2 activation phenotype in sporadic PRCC2. Transcriptome sequencing revealed the expression pattern of mutant alleles of NRF2, CUL3, and SIRT1 and also confirmed NRF2 activation in clinical specimens. Our results show a convergence in somatic mutations in sporadic PRCC2 with FH mutation in hereditary PRCC2. Cancer Res; 73(7); 2044–51. ©2013 AACR.
- National University of Singapore Singapore
- Translational Genomics Research Institute United States
- Spectrum Health United States
- Van Andel Institute United States
- Singapore General Hospital Singapore
Kelch-Like ECH-Associated Protein 1, Sequence Homology, Amino Acid, NF-E2-Related Factor 2, Gene Expression Profiling, Blotting, Western, Molecular Sequence Data, Intracellular Signaling Peptides and Proteins, 610, High-Throughput Nucleotide Sequencing, Cullin Proteins, Kidney Neoplasms, Immunoenzyme Techniques, Kidney Tubules, Proximal, Phenotype, Mutation, Biomarkers, Tumor, Humans, Amino Acid Sequence, Carcinoma, Renal Cell, Cells, Cultured, Oligonucleotide Array Sequence Analysis
Kelch-Like ECH-Associated Protein 1, Sequence Homology, Amino Acid, NF-E2-Related Factor 2, Gene Expression Profiling, Blotting, Western, Molecular Sequence Data, Intracellular Signaling Peptides and Proteins, 610, High-Throughput Nucleotide Sequencing, Cullin Proteins, Kidney Neoplasms, Immunoenzyme Techniques, Kidney Tubules, Proximal, Phenotype, Mutation, Biomarkers, Tumor, Humans, Amino Acid Sequence, Carcinoma, Renal Cell, Cells, Cultured, Oligonucleotide Array Sequence Analysis
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