Supplemental Figure 5. Lamp2a Alteration Did Not Affect Lysosomal Membrane protein levels.
Supplemental Figure 5. Lamp2a Alteration Did Not Affect Lysosomal Membrane protein levels.
Macroautophagy flux assays were performed using Lamp2a silenced and overexpressing cardiomyocytes. (A) Cardiomyocytes transfected with Lamp2a or control (Ctrl) siRNA were treated with or without Baf A1 and LAMP2a and LAMP1 protein levels determined. (B and C) The bar graphs show the densitometric analyses of the immunoblots. β-actin was used as a loading control. (D) Immunoblot analyses were performed to detect LAMP2a and LAMP1 protein levels in cardiomyocytes overexpressing Lamp2a. (E and F) The bar graphs show changes in the protein levels. β-actin was used as a loading control. Results are Mean ± SD from 4 independent groups. #(P<0.05) difference between infection within Veh groups; and %(P<0.05) difference between infection within Baf A1 groups.
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