Functional interdependence of the yeast SNF2, SNF5, and SNF6 proteins in transcriptional activation.
Copyright policy )Functional interdependence of the yeast SNF2, SNF5, and SNF6 proteins in transcriptional activation.
The SNF2, SNF5, and SNF6 genes of Saccharomyces cerevisiae are required for expression of a variety of differently regulated genes. Previous evidence implicated the SNF5 protein in transcriptional activation, and a DNA-bound LexA-SNF5 fusion protein was shown to activate expression of a nearby promoter. Here, we examine the functional relationship of the SNF2, SNF5, and SNF6 proteins. Activation by DNA-bound LexA-SNF5 fusion protein was greatly reduced in snf2 and snf6 mutants, indicating that activation by LexA-SNF5 requires SNF2 and SNF6 function. An spt6 mutation, which suppresses transcriptional defects caused by snf2, restored activation by LexA-SNF5 in a snf2 mutant. The SNF2 gene was sequenced and encodes a 194-kDa protein that is targeted to the nucleus. DNA-bound LexA-SNF2 fusion protein also activated transcription, dependent on SNF5 and SNF6. These findings suggest that SNF2, SNF5, and SNF6 function interdependently in transcriptional activation, possibly forming a heteromeric complex.
- Columbia University United States
- University Physicians United States
Adenosine Triphosphatases, Cell Nucleus, Saccharomyces cerevisiae Proteins, Base Sequence, Genotype, Transcription, Genetic, Recombinant Fusion Proteins, Genes, Fungal, Molecular Sequence Data, Restriction Mapping, Nuclear Proteins, Saccharomyces cerevisiae, beta-Galactosidase, DNA-Binding Proteins, Gene Expression Regulation, Fungal, Amino Acid Sequence, DNA, Fungal, Plasmids, Transcription Factors
Adenosine Triphosphatases, Cell Nucleus, Saccharomyces cerevisiae Proteins, Base Sequence, Genotype, Transcription, Genetic, Recombinant Fusion Proteins, Genes, Fungal, Molecular Sequence Data, Restriction Mapping, Nuclear Proteins, Saccharomyces cerevisiae, beta-Galactosidase, DNA-Binding Proteins, Gene Expression Regulation, Fungal, Amino Acid Sequence, DNA, Fungal, Plasmids, Transcription Factors
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