栽培种花生乙酰ACP硫酯酶基因(AhfatB)克隆及表达载体构建
Copyright policy ) null 李晓斐;
栽培种花生乙酰ACP硫酯酶基因(AhfatB)克隆及表达载体构建
本研究以花生栽培品种山花7号叶片DNA为模板进行PCR扩增，克隆获得花生乙酰ACP硫酯酶基因( AhfatB ) DNA序列，序列全长2 720 bp，编码区位于124 bp~2 341 bp，开放阅读框全长1 242 bp，编码413个氨基酸，基因内存在3个内含子分别位于607~754 bp、 878~1 495 bp和1 607~1 828 bp。提取花生幼果RNA，经RT-PCR扩增，得到 AhfatB 基因的全长cDNA，将其与PBI121表达载体35S启动子连接，替换其中的GUS基因，构建了植物表达载体PBI121- AhfatB 。
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These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
Citations provided by BIP!popularityPopularity provided by BIP!
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
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