High-resolution cryo-EM structures of TFIIH and their functional implications
Copyright policy )High-resolution cryo-EM structures of TFIIH and their functional implications
Eukaryotic transcription factor IIH (TFIIH) is a 500 kDa-multiprotein complex that harbors two SF2-family DNA-dependent ATPase/helicase subunits and the kinase activity of Cyclin-dependent kinase 7. TFIIH serves as a general transcription factor for transcription initiation by eukaryotic RNA polymerase II and plays an important role in nucleotide excision DNA repair. Aiming to understand the molecular mechanisms of its function and regulation in two key cellular pathways, the high-resolution structure of TFIIH has been pursued for decades. Recent breakthroughs, largely enabled by methodological advances in cryo-electron microscopy, have finally revealed the structure of TFIIH and its interactions in the context of the Pol II-pre-initiation complex, and provide a first glimpse of a TFIIH-containing assembly in DNA repair. Here, we review and discuss these recent structural insights and their functional implications.
- Howard Hughes Medical Institute United States
- QB3 United States
- Lawrence Berkeley National Laboratory United States
- University of California, Berkeley United States
Models, Molecular, DNA Repair, Transcription, Genetic, Protein Conformation, Cryoelectron Microscopy, Quantitative Structure-Activity Relationship, DNA, Xeroderma Pigmentosum Group A Protein, Humans, Transcription Factor TFIIH, Protein Binding
Models, Molecular, DNA Repair, Transcription, Genetic, Protein Conformation, Cryoelectron Microscopy, Quantitative Structure-Activity Relationship, DNA, Xeroderma Pigmentosum Group A Protein, Humans, Transcription Factor TFIIH, Protein Binding
selected citations These citations are derived from selected sources.This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).16 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 10% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Average impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
Citations provided by BIP!Popularity provided by BIP!