Correlation between Oxidative Stress and L-type Calcium Channel Expression in the Ventricular Myocardia of Selenium-deficient Mice
Copyright policy )Correlation between Oxidative Stress and L-type Calcium Channel Expression in the Ventricular Myocardia of Selenium-deficient Mice
Objective: Expression of the Cacna1c (calcium channel, voltage-dependent, Ltype, a1C subunit) gene was studied to investigate the relationship between oxidative stress and L-type calcium channels in the myocardium of seleniumdeficient mice. Methods: Selenium levels in liver and heart tissue samples from mice fed normal or selenium-deficientdiets were evaluated by fluorometry. In the same mice, glutathione peroxidase ( GPx) and Cacna1c gene expression were analysed, malondialdehyde (MDA) content and superoxide dismutase (SOD) activity were measured, oxidoreductase gene expression profiles were analysed (by DNA microarray), and myocardial structural changes were studied. Results: In selenium-deficient versus control mice, GPx expression and SOD activity were decreased, and Cacna1c expression and MDA concentration were increased. Selenoprotein oxidoreductase and nonselenoprotein oxidoreductase gene expression differed significantly between selenium-deficient and control mice. In selenium-deficient mice, myocardial fibres were separated by loose collagenous tissue; electron microscopy showed shortened sarcomeres, dilated sarcoplasmic reticulum, scattered myofibril lysis and increased mitochondria with amorphous matrix densities. Conclusion: L-type calcium channels were involved in selenium deficiency-induced cardiomyocyte injury, which was positively related to oxidative stress.
- Harbin Medical University China (People's Republic of)
Male, Calcium Channels, L-Type, Superoxide Dismutase, Heart Ventricles, Myocardium, Brain, Gene Expression, Kidney, Diet, Mice, Inbred C57BL, Mice, Oxidative Stress, Selenium, Liver, Animals, Female, Reactive Oxygen Species, Selenoproteins, Transcriptome, Oligonucleotide Array Sequence Analysis
Male, Calcium Channels, L-Type, Superoxide Dismutase, Heart Ventricles, Myocardium, Brain, Gene Expression, Kidney, Diet, Mice, Inbred C57BL, Mice, Oxidative Stress, Selenium, Liver, Animals, Female, Reactive Oxygen Species, Selenoproteins, Transcriptome, Oligonucleotide Array Sequence Analysis
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).16 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Average influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Average impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Average
Citations provided by BIP!Popularity provided by BIP!