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MOESM9 of Histone and DNA methylation control by H3 serine 10/threonine 11 phosphorylation in the mouse zygote

appsOther research productkeyboard_double_arrow_right Image 01 Jan 2017Publisher:FigshareFunded by:EC | BLUEPRINT, DFG | unidentified

Authors: Lan, Jie; Lepikhov, Konstantin; Giehr, Pascal; Joern Walter;

doi: 10.6084/m9.figshare.c.3690569_d2 , 10.6084/m9.figshare.c.3690569_d2.v1

MOESM9 of Histone and DNA methylation control by H3 serine 10/threonine 11 phosphorylation in the mouse zygote

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Abstract

Additional file 9. Effects of H3.1/2/3-GFPT11A co-expression with G9amCat-NLS-GFP in mouse zygotes on H3K9me2. a Shown are the representative images of PN4/5 stage zygotes stained with antibodies against H3K9me2. DNA is visualized by DAPI. m Maternal pronucleus, p paternal pronucleus, pb polar body. Scale bar 50 µm. b Quantification of H3K9me2 signals, normalized against DNA signals in both parental genomes of zygotes at PN4/5. Relative signal intensities in control groups are set to 1. Statistical significance was calculated using t test (***P

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Histone and DNA methylation control by H3 serine 10/threonine 11 phosphorylation in the mouse zygote
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