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USF2/FIP Associates with the b-Zip Transcription Factor, c-Maf, via Its bHLH Domain and Inhibits c-Maf DNA Binding Activity

descriptionPublicationkeyboard_double_arrow_right Article 01 Feb 1997 English Publisher:Elsevier BVJournal:Biochemical and Biophysical Research Communications, volume 231, pages 333-339 (issn: 0006-291X,

Authors: Cornelia Kurschner; James I. Morgan;

doi: 10.1006/bbrc.1997.6097 , 10.1006/bbrc.1997.6393

pmid: 9070273

USF2/FIP Associates with the b-Zip Transcription Factor, c-Maf, via Its bHLH Domain and Inhibits c-Maf DNA Binding Activity

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Abstract

In screening for proteins that interact with the basic zipper (bZip) transcription factor, c-Maf, we isolated USF2/FIP. USF2 is a member of the bHLH-Zip protein family, possessing a basic (b) DNA binding region, a helix-loop-helix (HLH) motif, and a leucine zipper (Zip) structure. Mutants of USF2 that lacked a Zip formed heterodimers with c-Maf, but did not homodimerize. Deletion of the USF2 basic region or mutation of its helices abrogated its binding to c-Maf, but had no effect on homodimerization. A functional c-Maf bZip motif was necessary for both homodimerization and heterodimerization with USF2. These data suggest a tetrameric configuration for Maf-USF2 complexes. In the presence of USF2, the DNA binding activity of c-Maf was markedly reduced. Therefore, USF2 and c-Maf may interact to regulate gene expression.

Related Organizations

St. Jude Children's Research Hospital
United States

Keywords

Leucine Zippers, Binding Sites, Kainic Acid, Helix-Loop-Helix Motifs, Nerve Tissue Proteins, Recombinant Proteins, DNA-Binding Proteins, Basic-Leucine Zipper Transcription Factors, Biopolymers, G-Box Binding Factors, Proto-Oncogene Proteins, Proto-Oncogene Proteins c-maf, Upstream Stimulatory Factors, Cycloheximide, Promoter Regions, Genetic, Protein Binding, Transcription Factors

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Transcription factor Maf
2017IsRelatedTo
AltName: Full=Upstream transcription factor 2;
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Upstream stimulatory factor 2
2017IsRelatedTo

Impact byBIP!

	citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	25
	popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.	Average
	influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	Top 10%
	impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.	Top 10%