
The analytical performance of a new automated HPLC system, for the determination of HbA1C in blood (Tosoh HLC-723 G7), was studied.The study design included the evaluation of imprecision, linearity, interference and carryover. Comparison study was performed by comparing HbA1C results with those obtained from an established method (Bio-Rad Variant II).Total imprecision was less than 1.34% and the results were linear up to 17.2% HbA1C. The method showed a wide analytical range, and no carryover between specimens. Comparison study yielded, r=0.989, Sy.x=0.255, regression equation (y=0.9895x-0.35); Bland-Altman plot showed a mean bias=- 0.43% HbA1C with confidence limits ranging from -0.48% to -0.38% HbA1C. The presence of abnormal hemoglobin was clearly revealed, and no interference from labile HbA1C was apparent.The HLC-723 G7 instrument seems to be a reliable system for routine assay of HbA1C.
Glycated Hemoglobin, Hemoglobins, Abnormal, Humans, Bilirubin, Chromatography, High Pressure Liquid, Triglycerides
Glycated Hemoglobin, Hemoglobins, Abnormal, Humans, Bilirubin, Chromatography, High Pressure Liquid, Triglycerides
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