Structure of human lanthionine synthetase C-like protein 1 and its interaction with Eps8 and glutathione
Copyright policy )Structure of human lanthionine synthetase C-like protein 1 and its interaction with Eps8 and glutathione
Eukaryotic lanthionine synthetase C-like protein 1 (LanCL1) is homologous to prokaryotic lanthionine cyclases, yet its biochemical functions remain elusive. We report the crystal structures of human LanCL1, both free of and complexed with glutathione, revealing glutathione binding to a zinc ion at the putative active site formed by conserved GxxG motifs. We also demonstrate by in vitro affinity analysis that LanCL1 binds specifically to the SH3 domain of a signaling protein, Eps8. Importantly, expression of LanCL1 mutants defective in Eps8 interaction inhibits nerve growth factor (NGF)-induced neurite outgrowth, providing evidence for the biological significance of this novel interaction in cellular signaling and differentiation.
- Oklahoma Medical Research Foundation United States
- University of Oklahoma United States
- University of Toledo United States
- Chinese Academy of Sciences China (People's Republic of)
- Institute of Biophysics China (People's Republic of)
Models, Molecular, Neurons, Intracellular Signaling Peptides and Proteins, Glutathione, PC12 Cells, Recombinant Proteins, Protein Structure, Tertiary, Rats, Receptors, G-Protein-Coupled, Zinc, Gene Expression Regulation, Mutation, Nerve Growth Factor, Escherichia coli, Neurites, Animals, Humans, Adaptor Proteins, Signal Transducing, Protein Binding, Signal Transduction
Models, Molecular, Neurons, Intracellular Signaling Peptides and Proteins, Glutathione, PC12 Cells, Recombinant Proteins, Protein Structure, Tertiary, Rats, Receptors, G-Protein-Coupled, Zinc, Gene Expression Regulation, Mutation, Nerve Growth Factor, Escherichia coli, Neurites, Animals, Humans, Adaptor Proteins, Signal Transducing, Protein Binding, Signal Transduction
selected citations These citations are derived from selected sources.This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).61 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 10% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 10% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
Citations provided by BIP!Popularity provided by BIP!