Description A set of 91 example audio recordings and a .csv file with associated metadata to accompany Bradfer-Lawrence et al. (2024) "The Acoustic Index User’s Guide: A practical manual for defining, generating and understanding current and future acoustic indices" Methods in Ecology & Evolution, and the online app accessible at https://ecohack.shinyapps.io/Acoustic_Index_Users_Guide/ Sixty-six recordings were collected in air, water, and soil, from sites around the world. They include a wide range of soundscape pattens, reflecting diverse sources of sound including biophony, geophony, and anthropophony. The recordings were collected with a range of equipment and sample rates; full details are available in the metadata file. We also include 25 synthetic soundscapes created with Audacity software. Collectively, these offer users the opportunity to explore a variety of soundscapes and cultivate their understanding of the links between acoustic patterns and indices values.