Cdk1 Triggers Association of RNA Polymerase to Cell Cycle Promoters Only after Recruitment of the Mediator by SBF
Copyright policy )Cdk1 Triggers Association of RNA Polymerase to Cell Cycle Promoters Only after Recruitment of the Mediator by SBF
Activation of HO in yeast involves recruitment of transcription factors in two waves. The first is triggered by inactivation of Cdk1 at the end of mitosis, which promotes import into the nucleus of the Swi5 transcription factor. Swi5 recruits the Swi/Snf chromatin-remodeling complex, which then facilitates recruitment of the SAGA histone acetylase, which in turn permits the binding of the SBF transcription factor. We show here that SBF then recruits the SRB/mediator complex and that this process occurs in the absence of Cdk1 activity. The second wave is triggered by reactivation of Cdk1, which leads to recruitment of PolII, TFIIB, and TFIIH. RNA polymerase is, therefore, recruited to HO in two steps and not as a holoenzyme. A similar sequence of events occurs at other SBF-regulated promoters, such as CLN1, CLN2, and PCL1.
TATA-Binding Protein Associated Factors, Saccharomyces cerevisiae Proteins, Cell Cycle, Cell Cycle Proteins, Cell Biology, DNA-Directed RNA Polymerases, Protein Serine-Threonine Kinases, Cyclin-Dependent Kinases, DNA-Binding Proteins, Fungal Proteins, Transcription Factors, TFII, Yeasts, CDC2 Protein Kinase, Transcription Factor TFIIB, Transcription Factor TFIID, Deoxyribonucleases, Type II Site-Specific, Promoter Regions, Genetic, Molecular Biology, Transcription Factor TFIIH, DNA Polymerase III, Transcription Factors
TATA-Binding Protein Associated Factors, Saccharomyces cerevisiae Proteins, Cell Cycle, Cell Cycle Proteins, Cell Biology, DNA-Directed RNA Polymerases, Protein Serine-Threonine Kinases, Cyclin-Dependent Kinases, DNA-Binding Proteins, Fungal Proteins, Transcription Factors, TFII, Yeasts, CDC2 Protein Kinase, Transcription Factor TFIIB, Transcription Factor TFIID, Deoxyribonucleases, Type II Site-Specific, Promoter Regions, Genetic, Molecular Biology, Transcription Factor TFIIH, DNA Polymerase III, Transcription Factors
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).121 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 10% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 10% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 1%
Citations provided by BIP!Popularity provided by BIP!