Copyright information:Taken from "Regulation of histone H3K4 tri-methylation and PAF complex recruitment by the Ccr4-Not complex"Nucleic Acids Research 2007;35(7):2428-2439.Published online 28 Mar 2007PMCID:PMC1874646.© 2007 The Author(s) () Schematic representation of the locus and the amplicons used in (B–H). () ChIP analysis of the ORF in BY4741 and Δ strains. Exponentially growing cells were subjected to ChIP analysis using H3K4me3 antibodies. () As in (A), using H3K4me2 antibodies. () As in (A), using H3K36me2 antibodies. () As in (A), using H3K79me2 antibodies. () As in (A), using CTD antibodies (8WG16). () Quantitative reverse-transcriptase PCR analysis of and mRNA levels in BY4741 WT and Δ strains. () ChIP analysis of H3K4me3 levels in MY1 WT and Δ cells. () ChIP analysis of H3K4me2 levels in MY1 WT and Δ cells. () Not4p is recruited to the 5′ region of the ORF. Strains expressing mycAVI-tagged Not4p were transformed with a BirA expression plasmid and subjected to ChIP analysis. Signals were normalized to an empty plasmid control.