Evidence of an association between poly(3-hydroxybutyrate) accumulation and phosphotransbutyrylase expression in Bacillus megaterium
Copyright policy )Evidence of an association between poly(3-hydroxybutyrate) accumulation and phosphotransbutyrylase expression in Bacillus megaterium
Molecular analysis of a genomic region of Bacillus megaterium, a polyhydroxybutyrate (PHB)-producing microorganism, revealed the presence of a gene coding for the enzyme phosphotransbutyrylase (Ptb). Enzyme activity was measured throughout the different growth phases of B. megaterium and was found to correlate with PHB accumulation during the late-exponential growth phase. Ptb expression was repressed by glucose and activated by the branched amino acids isoleucine and valine. Overexpression of Act(Bm), a sigma(54) regulator from B. megaterium whose gene is located upstream from ptb, caused an increase in Ptb activity and PHB accumulation in B. megaterium.
- Brookhaven National Laboratory United States
- University of Buenos Aires Argentina
Polyesters, Carbohydrates, Hydroxybutyrates, Sigma Factor, DNA-Directed RNA Polymerases, DNA-Binding Proteins, Kinetics, Phosphate Acetyltransferase, Bacterial Proteins, Genes, Bacterial, Bacillus megaterium, RNA Polymerase Sigma 54, Amino Acids, Branched-Chain
Polyesters, Carbohydrates, Hydroxybutyrates, Sigma Factor, DNA-Directed RNA Polymerases, DNA-Binding Proteins, Kinetics, Phosphate Acetyltransferase, Bacterial Proteins, Genes, Bacterial, Bacillus megaterium, RNA Polymerase Sigma 54, Amino Acids, Branched-Chain
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