Copyright information:Taken from "Assaying protein palmitoylation in plants"http://www.plantmethods.com/content/4/1/2Plant Methods 2008;4():2-2.Published online 11 Jan 2008PMCID:PMC2244627.int mutant TIP1 CA, were put through the biotin switch protocol summarised in Figure 1. Samples were treated with (Hyd+) or without (Hyd-) the thioester cleavage reagent hydroxylamine. The loading controls show that equal amounts of TIP1 and TIP1 CA were loaded onto neutravidin beads. The lanes labelled 'S-acylation' show the levels of TIP1 and TIP1 CA recovered from the neutravidin beads, and therefore originally S-acylated. TIP1 and TIP1 CA have masses of 70 kDa. The results show that Arabidopsis TIP1 binds acyl groups by a covalent thioester bond but TIP1 CA does not, consistent with previous results using a H-palmitic acid S-acylation assay [18].