The Monod or hyperbolic kinetic formulation became the de facto kinetic descriptor in activated sludge models in the '80s-'90s. It still plays a prominent role in activated sludge models; however, a dual hyperbolic formulation (with respect to both substrate and active biomass) that implicitly accommodates storage as well as more rationally describes metabolic kinetics is more prominent in governing metabolic functions in activated sludge models (ASMs) promulgated by working groups under the aegis of the International Water Association (IWA). There are other kinetic formulations used in the models of this group as well as models of the activated sludge process formulated by others. There is lack of consistency in the choice of kinetic formulations for various processes even within the IWA family of models. This thesis examined the basis for the common Monod and dual-hyperbolic formulations and puts forward theoretical justifications of them based on various considerations of mass transfer, storage and metabolic rate formulation. Other models were developed based on major governing principles. Models used in biological wastewater treatment are Eulerian gross descriptors of a process involving mass transfer, many substrates, and metabolic pathways and their enzymes, contained within many microorganisms. Any model at this level is merely a fit of mathematical formulations to data. As number of processes in biotreatment models increases along with mathematical descriptors and their associated coefficients, fitting an overall model improves to a point. But beyond this there has been little justification of the Monod or other kinetic expressions. Since a variety of formulations have been proposed for two of the primary metabolic processes which are hydrolysis of complex substrates and then metabolism of resulting readily degradable (or simple) substrates, the primary objective of this study was to examine these processes by experiment and determine the most appropriate models for each step. A chemostat was used in this study to culture active biomass acclimatized to a feed containing starch, a complex substrate requiring hydrolysis, and glucose which is readily biodegradable and also a product of starch hydrolysis. Active mass samples were taken from the chemostat and placed in batch reactors where varying concentrations of active mass were exposed to varying concentrations of either glucose or starch. Before adding any substrate to the batch reactors the active mass was aerated for a period of time until DO change was not observed to ensure that any stored or extraneous substrate was metabolized. After addition of one or the other substrate, the rate of dissolved oxygen (DO) uptake was monitored over the initial 15 minutes in the batch culture. Rapidly changing conditions dictated the necessity of using DO as a surrogate for either starch or glucose. Over 249 models were examined for their ability to describe glucose metabolism for 16 different batch experiments. The Monod model gave a good fit to the data. Other models that were equally applicable were too complex or made no scientific sense. Another series of batch tests were conducted using only starch as a substrate. Again substrate concentration and active mass concentration were varied in the batch tests in six different batch runs. The results from these experiments were again used to determine the adequacy of 249 starch hydrolysis models. The Monod type relationship and a dual hyperbolic relation again proved to be the most reasonable choices. Also a first-order model based on starch concentration was applicable. However no general set of coefficients that applied to all experiments was found for any model; it is necessary to calibrate either model to environmental conditions. All of these models are fairly simple in terms of concept as well as determination of parameters and have scientific sensibility in describing hydrolysis of SBCOD. Keywords: wastewater, activated sludge, dissolved oxygen, modeling