Regulation of alternative splicing in Drosophila by 56 RNA binding proteins
Copyright policy )Regulation of alternative splicing in Drosophila by 56 RNA binding proteins
Alternative splicing is regulated by RNA binding proteins (RBPs) that recognize pre-mRNA sequence elements and activate or repress adjacent exons. Here, we used RNA interference and RNA-seq to identify splicing events regulated by 56 Drosophila proteins, some previously unknown to regulate splicing. Nearly all proteins affected alternative first exons, suggesting that RBPs play important roles in first exon choice. Half of the splicing events were regulated by multiple proteins, demonstrating extensive combinatorial regulation. We observed that SR and hnRNP proteins tend to act coordinately with each other, not antagonistically. We also identified a cross-regulatory network where splicing regulators affected the splicing of pre-mRNAs encoding other splicing regulators. This large-scale study substantially enhances our understanding of recent models of splicing regulation and provides a resource of thousands of exons that are regulated by 56 diverse RBPs.
- Lawrence Berkeley National Laboratory United States
- Dana-Farber Cancer Institute United States
- Harvard University United States
- University of California, Berkeley United States
- Department of Molecular Biology and Biochemistry University of California United States
Resource, 570, TATA-Binding Protein Associated Factors (mesh), Bioinformatics, 1.1 Normal biological development and functioning, 3102 Bioinformatics and Computational Biology (for-2020), Heterogeneous-Nuclear Ribonucleoproteins (mesh), Bioinformatics and Computational Biology, 11 Medical and Health Sciences (for), Medical and Health Sciences, 3105 Genetics (for-2020), Heterogeneous-Nuclear Ribonucleoproteins, Drosophila Proteins (mesh), Genetics, RNA Precursors, Animals (mesh), Animals, Drosophila Proteins, 1.1 Normal biological development and functioning (hrcs-rac), TATA-Binding Protein Associated Factors, 31 Biological Sciences (for-2020), Drosophila (mesh), Genetics (rcdc), Sequence Analysis, RNA, Generic health relevance (hrcs-hc), RNA Precursors (mesh), RNA-Binding Proteins, Alternative Splicing (mesh), Exons, Biological Sciences, RNA-Binding Proteins (mesh), 06 Biological Sciences (for), Alternative Splicing, Bioinformatics (science-metrix), RNA, RNA (mesh), Drosophila, RNA Interference, Generic health relevance, Exons (mesh), RNA Interference (mesh), Sequence Analysis
Resource, 570, TATA-Binding Protein Associated Factors (mesh), Bioinformatics, 1.1 Normal biological development and functioning, 3102 Bioinformatics and Computational Biology (for-2020), Heterogeneous-Nuclear Ribonucleoproteins (mesh), Bioinformatics and Computational Biology, 11 Medical and Health Sciences (for), Medical and Health Sciences, 3105 Genetics (for-2020), Heterogeneous-Nuclear Ribonucleoproteins, Drosophila Proteins (mesh), Genetics, RNA Precursors, Animals (mesh), Animals, Drosophila Proteins, 1.1 Normal biological development and functioning (hrcs-rac), TATA-Binding Protein Associated Factors, 31 Biological Sciences (for-2020), Drosophila (mesh), Genetics (rcdc), Sequence Analysis, RNA, Generic health relevance (hrcs-hc), RNA Precursors (mesh), RNA-Binding Proteins, Alternative Splicing (mesh), Exons, Biological Sciences, RNA-Binding Proteins (mesh), 06 Biological Sciences (for), Alternative Splicing, Bioinformatics (science-metrix), RNA, RNA (mesh), Drosophila, RNA Interference, Generic health relevance, Exons (mesh), RNA Interference (mesh), Sequence Analysis
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