
This protocol demonstrates how to map strand-oriented long reads to a genome, and visualise them in a genome browser. The general idea is to use minimap2 to create stranded BAM files, which are split for forward/reverse orientation then converted into BigWig format for display in a genome browser. Input(s): stranded fastq files (see protocol Preparing Reads for Stranded Mapping) a FASTA file containing the genome / sequence of interest. Output(s): Genome-mapped stranded BAM files Genome-mapped stranded BigWig files
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