Avaliou-se, in vitro, a capacidade seladora, do MTA-Angelus, ProRoot-MTA, Super-EBA e MBP-c, quando utilizados para selar perfurações na região de furca de molares humanos extraídos; quando aplicados isoladamente ou em associação com uma matriz de gesso Paris, aplicada no fundo do trajeto da perfuração. O selamento produzido pelos materiais foi analisado por meio da infiltração do corante rhodamine B 0,2%. A análise estatística aplicada aos dados obtidos demonstrou que, quando utilizado isoladamente (sem matriz), o cimento MBP-c apresentou os menores índices de infiltração marginal, seguido pelo Super-EBA, apresentando diferença estatística significante (pIt has been evaluated in vitro the sealing capacity of MTA-Angelus, ProRoot-MTA, Super-EBA and MBP-c when sealing furcal perforations of extracted human molars. The experiment took place with materials both isolated as well as in association with a plaster of Paris matrix applied at the bottom of the perfuration path. The materials sealing capacity was analyzed by dye leakage of rhodamine B 0,2%.The statistical analysis revealed that when isolated and tested (without the matrix), the cement MBP-c presented the smallest coefficient of marginal leakage, followed by the Super-EBA. There was a statistical significance between those and the cements ProRoot - MTA e MTA - Angelus (p<0,001). In the presence of the matrix, the cement MBP-c also had a superior performance having statistical significance between those and the cements ProRoot - MTA e MTA - Angelus (p<0,05); The Plaster of Paris matrix had a negative impact with statistical significance regarding the sealing capacity of the cements Super-EBA and the MBP-c (p<0,05). Nevertheless, it avoided the leakage of the respective sealing materials.