A small fraction of dermatan sulfate with significantly increased anticoagulant activity was selected by interaction with the first complement protein
A small fraction of dermatan sulfate with significantly increased anticoagulant activity was selected by interaction with the first complement protein
Dermatan sulfate (DS) is a member of the family of structurally complex, sulfated, linear heteropolysaccharides called glycosaminoglycans (GAGs). It has a similar structure to heparin and heparan sulfate (HS), but with acetylgalactosamine replacing glucosamine, and the uronic acid moiety, mainly iduronic, joined 1→3 to the hexosamine. We are studying the relationships between structure and activities of dermatan sulfate, in particular those associated with the thrombin inhibition mediated by heparin cofactor II (HCII). As we have demonstrated with heparin, a small fraction of dermatan sulfate was isolated by precipitation with the first component of the complement system, under very specific conditions of low ionic strength, and the presence of calcium ions. The sulfate content and the anticoagulant activity of the dermatan sulfate fraction isolated in the precipitate were three and four times greater respectively than the starting material. Our in vivo studies showed that this fraction has threefold higher thrombolytic activity than the DS. All these results suggest that this fraction could be used as a therapeutic agent for thrombi dissolution. © 2004 Elsevier Ltd. All rights reserved.
Fil: Alberto, Maria Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Calabrese, Graciela Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Ciencias Biológicas; Argentina
Fil: Lazzari, María Ángela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Marani, Mariela Mirta. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Ciencias Biológicas; Argentina
Fil: Tubio, Maria Rosario. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Ciencias Biológicas; Argentina
Fil: Fernández De Recondo, Marta E.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Ciencias Biológicas; Argentina
Fil: Recondo, Eduardo F.. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Gag, Molecular Interactions, Antithrombin, Complement System, Con A, Dermatan Sulfate, Hs, C1, Glycosaminoglycan, Free Ds, The First Protein Complex Of the Complement System, https://purl.org/becyt/ford/3.1, Concanavalin A, Free Dermatan Sulfate, Heparin Cofactor Ii, At, https://purl.org/becyt/ford/3, Ds
Gag, Molecular Interactions, Antithrombin, Complement System, Con A, Dermatan Sulfate, Hs, C1, Glycosaminoglycan, Free Ds, The First Protein Complex Of the Complement System, https://purl.org/becyt/ford/3.1, Concanavalin A, Free Dermatan Sulfate, Heparin Cofactor Ii, At, https://purl.org/becyt/ford/3, Ds
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