In recent years, there is a growing interest in the role of PPARγ agonists in skin biology. Specifically, we have previously shown that agonistic PPARγ modulators affect hair follicle growth and function. PPARγ-mediated signalling has also been implicated in the regulation of mitochondrial energy metabolism in several tissues (adipose and brain), but how PPARγ-stimulation affects mitochondrial biology in the skin is still unknown. In this study, we aimed to explore the effects of PPARγ-stimulation in organ cultured human hair follicles. Overall design: 25 freshly isolated HFs from two females were treated with 0.01 mM GMG-43AC (provided by Giuliani SpA, Milan, Italy) or vehicle for 6h.