For stomata1 density and length, a preliminary survey of 40 grapevines and its wild relatives, Muscadinia rotundifolia was conducted. Within genus Vitis, the stomata1 density and length of the diploid cultivars ranged between 140 and 300/mm^2 and between 20 and 30 pm, respectively. On the other hand, the stomata1 density and length of the tetraploid cultivars ranged between 80 and 120/mm^2 and between 32 and 37 pm, respectively. Stomata1 density of relatives of gemrs Vitis, Muscadinia rotundifolia was about 400/mm^2, and the stomata1 length was about 19 μm. These results suggest that genetic differentiation in stomata1 density and length may occurred within family Vitaceue.