The objective of this study was to determine if the original bamboo vinegar, collected from six different temperatures (80-150, over 80, 90-92, 99-102, 120-123 and 145-150℃), obtained from Moso bamboo (Phyllostachys heterocycla), combined with a specific duration of soaking or vacuum treatment, could be used to increase the fungi resistance of bamboo. The results indicated that the absorption of bamboo vinegar was between 0.89 to 23.38mg/cm^3. Also, the amount of bamboo vinegar absorptions by the different bamboo specimens increased with the amount of treatment time, regardless of the type of treatment or the pre-processed bamboo material. From the results of SEM and Marco-observations, the growth rate of Aspergillus flavus, Aspergillus niger and airborne fungi particles for the collected bamboo vinegar at 120-123℃ was about the range of 0-10% after being inoculated for over 70 days, but it was ineffective, 100% of fungal colony growth, for the control specimens after 5 days. For the bamboo vinegar collected at a temperature of 80-150℃, the airborne fungi particles and Trichoderma viride were 10-30% and 50-90% respectively. However, after 70 days the growth rate of Aspergillus flavus days was higher than any of the others, except for the bamboo vinegar collected at 120-123℃. The above results show that the fungi resistance of bamboo materials is influenced by the temperature of the bamboo vinegar.