Fas receptor expression on asthmatic eosinophils.
Copyright policy )Fas receptor expression on asthmatic eosinophils.
Activation of the Fas receptor (Fas; CD95) on eosinophils induces apoptosis and may play a role in controlling eosinophil number in blood and tissue. To determine whether Fas expression on eosinophils is different between asthmatics and non-asthmatics, eosinophils were isolated from the peripheral blood using a three-step procedure of dextran sedimentation, ficoll hypaque separation and negative selection with anti-CD16 monoclonal antibody bound to magnetic beads. Fas expression was quantified by flow cytometry. Fas expression and the percentage of eosinophils expressing Fas were not different between asthmatics (n=9) and non-asthmatics (n=14).
- University of Arizona College of Medicine- Phoenix United States
Adult, Adolescent, Antibodies, Monoclonal, In Vitro Techniques, Middle Aged, Asthma, Eosinophils, Receptors, Antigen, Humans, fas Receptor, Aged
Adult, Adolescent, Antibodies, Monoclonal, In Vitro Techniques, Middle Aged, Asthma, Eosinophils, Receptors, Antigen, Humans, fas Receptor, Aged
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