
A kaseini-broth enrichment medium (KBEM) for the isolation improvement of Campylobacter jejuni-Campylobacter coli from stool samples is described. Isolation of Campylobacters from stool specimens by direct inoculation onto solid selective medium was compared with isolation after twenty-four hours enrichment at 37 degrees C in KBEM, followed by subculture onto the same solid selective medium. Of 156 examined stool samples from diarrhoeal children positive results were obtained from 17 patients altogether: 11 by direct inoculation on selective media and 6 only owing to enrichment. Thus, an increase of 35.3% in the isolation rate was obtained by using the enrichment medium. The same medium allows the preservation of isolates of Campylobacter jejuni-Campylobacter coli for 2 months and longer at 37 degrees C.
culture media, child, Bacteriological Techniques, culture medium, Time Factors, isolation and purification, growth, development and aging, microbiology, MeSH Bacteriological Techniques, Campylobacter coli, Culture Media, Campylobacter jejuni, Feces, Humans, Time Factors EMTREE medical terms article, human, microbiological examination, Child, comparative study, time
culture media, child, Bacteriological Techniques, culture medium, Time Factors, isolation and purification, growth, development and aging, microbiology, MeSH Bacteriological Techniques, Campylobacter coli, Culture Media, Campylobacter jejuni, Feces, Humans, Time Factors EMTREE medical terms article, human, microbiological examination, Child, comparative study, time
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