The possibility of establishing the etiological and epidemiological importance of Escherichia on the basis of the determination of their persistence factors made it necessary to develop simple and, at the same time, informative methods for their indication. For this purpose a new selective medium for the isolation of Escherichia with persistence properties was developed and approved. The use of this medium permits the early detection of persistent strains in the process of their isolation from a human body and environmental objects. The nutrient medium may be used in clinics, as well as in sanitary and hygienic practice, especially in the system of microbiological screening studies.