The route of immunization, dose and nature of antigens are relevant for induction of specific immune responses but they are particularly influenced by the state of immunomaturation of fish. It is difficult, however, to determine when the fish immune system has matured and, accordingly, when the fry can be successfully vaccinated. To establish some general parameters which can correlate the development of protective immunity with maturation of the immune system we review: (i) the appearance of first lympho-haemopoietic cell precursors; (ii) the histological and functional maturation of lymphoid organs; (iii) the relevance of phagocytosis and transport of maternal Ig to eggs for early vaccination; (iv) the appearance of both T-like and B-like cells and their correlation with the development of the cell-mediated and humoral immune responses and (v) the mechanisms which may be involved in the induction of tolerance following early immunization of fry. These data rather than a general rule for vaccination of larval fish indicate that the earliest age (size) that a fish can be vaccinated differs between species and vaccines.