Antibacterial immune response in Astacus leptodactylus (Crustacea, Decapoda).
Copyright policy )Antibacterial immune response in Astacus leptodactylus (Crustacea, Decapoda).
Antibacterial substances (ABS) in the hemolymph of Astacus leptodactylus were studied by agar-plate lysis and inhibition tests. The results demonstrate ABS against Gram-positive and Gram-negative bacteria. ABS against Gram-positive bacteria were identified as a lysozyme with a molecular weight of 14 kDa, heat resistance (< 50 degrees C) and lability to freezing. ABS against Gram-negative bacteria lost 50% of their activity at 50 degrees C and completely at 70 degrees C but were stable to freezing. Both ABS are inducible; they are produced and stored in the granular and semigranular hemocytes. Lysozyme is found in treated and untreated animals, ABS against Gram-negative bacteria only after induction.
Hot Temperature, Astacoidea, Bacterial Infections, Hydrogen-Ion Concentration, Hemolymph, Freezing, Gram-Negative Bacteria, Animals, Electrophoresis, Polyacrylamide Gel, Immunization, Muramidase
Hot Temperature, Astacoidea, Bacterial Infections, Hydrogen-Ion Concentration, Hemolymph, Freezing, Gram-Negative Bacteria, Animals, Electrophoresis, Polyacrylamide Gel, Immunization, Muramidase
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