Interleukin (IL) 4, the cytokine that isn't there: reactivity of IL-4 antibodies with cells in IL-4 -/- mice.
Copyright policy )Interleukin (IL) 4, the cytokine that isn't there: reactivity of IL-4 antibodies with cells in IL-4 -/- mice.
We analyzed cytokine expression in recipient spleens and cardiac allografts placed in mice that were unable to synthesize interleukin (IL)-4 due to disruption of the IL-4 gene (IL-4 -/-) and in wild-type (IL-4 +/+) mice. Polyclonal BL-4P and monoclonal 11B11, 1D11, and 24G2 anti-IL-4 antibodies were used to detect cell-surface and cytoplasmic antigens in sections of frozen tissue. All of the antibodies were found to react with non-IL-4 determinants associated with graft-infiltrating cells, and BL-4P, 1D11, and 24G2 bound to cells and connective tissue in the spleens of IL-4 -/- mice. The IL-4-producing cell line, X63Ag8-653 (X63), was used as a positive control for IL-4 staining and to test the ability of recombinant IL-4 to block the binding of antibodies to IL-4.
- Melbourne Health Australia
- Royal Melbourne Hospital Australia
Mice, Knockout, Mice, Inbred BALB C, Graft Survival, Antibodies, Monoclonal, Mice, Inbred Strains, Polymerase Chain Reaction, Antibodies, Cell Line, Immunoenzyme Techniques, Mice, Inbred C57BL, Mice, CD4 Antigens, Animals, Cytokines, Heart Transplantation, Transplantation, Homologous, Interleukin-4, Lymphocytes, Spleen
Mice, Knockout, Mice, Inbred BALB C, Graft Survival, Antibodies, Monoclonal, Mice, Inbred Strains, Polymerase Chain Reaction, Antibodies, Cell Line, Immunoenzyme Techniques, Mice, Inbred C57BL, Mice, CD4 Antigens, Animals, Cytokines, Heart Transplantation, Transplantation, Homologous, Interleukin-4, Lymphocytes, Spleen
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