
An immunoperoxidase assay for the serological diagnosis of Babesia bigemina was developed. The antigen slides were prepared from B. bigemina-infected blood and stored at -20 degrees C. One hundred and sixty five sera were tested, comparing the immunoperoxidase assay to the indirect fluorescent antibody test. A coincidence of 95% was observed between both tests. For the immunoperoxidase assay, a relative sensitivity of 94.8%, a relative specificity of 95.5%, and a positive predictive value of 96.8% were calculated. The results demonstrated the efficacy of this technique for detecting antibodies to B. bigemina.
Immunoenzyme Techniques, Babesiosis, Animals, Cattle Diseases, Reproducibility of Results, Cattle, Sensitivity and Specificity
Immunoenzyme Techniques, Babesiosis, Animals, Cattle Diseases, Reproducibility of Results, Cattle, Sensitivity and Specificity
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