Dot-ELISA for human leptospirosis employing immunodominant antigen.
Copyright policy )Dot-ELISA for human leptospirosis employing immunodominant antigen.
A dot enzyme-linked immunosorbent assay which uses a proteinase-K resistant antigen (PK-Dot-ELISA) to detect antileptospiral IgM antibodies was compared to the microscopic agglutination test (MAT). The assay was evaluated in serum samples from patients with leptospirosis (n = 89), typhoid fever (n = 10), malaria (n = 19), syphilis (n = 20), hepatitis (n = 16) and clinically healthy individuals (n = 92). The PK-Dot-ELISA presented a sensitivity of 92.1% and a specificity of 97.5%. The overall results of the PK-Dot-ELISA were similar to those of the MAT. However, the PK-Dot-ELISA was capable of detecting antibody activity in 43% of acute-phase sera which were negative by the MAT. Our data suggest that PK-Dot-ELISA can be used as an important portable field serodiagnostic assay for acute leptospirosis.
- Instituto Adolfo Lutz Brazil
Leptospira, Antigens, Bacterial, Immunodominant Epitopes, Enzyme-Linked Immunosorbent Assay, Antibodies, Bacterial, Sensitivity and Specificity, Hepatitis, Immunoglobulin M, Evaluation Studies as Topic, Agglutination Tests, Humans, Leptospirosis, Serologic Tests, Syphilis, Malaria, Falciparum, Typhoid Fever
Leptospira, Antigens, Bacterial, Immunodominant Epitopes, Enzyme-Linked Immunosorbent Assay, Antibodies, Bacterial, Sensitivity and Specificity, Hepatitis, Immunoglobulin M, Evaluation Studies as Topic, Agglutination Tests, Humans, Leptospirosis, Serologic Tests, Syphilis, Malaria, Falciparum, Typhoid Fever
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