At different locality in Egypt, free living amoebae (FLA) were detected in 32%, 20% and 4% of water samples obtained from swimming pools, surface water canals and tap water respectively. Naegleria spp. were identified from 12 (75%) out of 16 swimming pool samples and 6 (60%) out of 10 surface water & canals samples from different Governorates in Egypt, while Acanthamoeba spp. were identified from 4 (25%) of 16 swimming pools samples, 4 (40%) out of 10 surface water & canals samples and 2 (100%) tap samples. The isolated FLA in the present study were non-pathogenic as proved by the pathogenicity test.