[The isolation and characteristics of monoclonal antibodies to recombinant proteins of HIV-1 and HIV-2--the env and gag gene products].
Copyright policy )[The isolation and characteristics of monoclonal antibodies to recombinant proteins of HIV-1 and HIV-2--the env and gag gene products].
Ten hybridomas secreting monoclonal antibodies (Mab) against recombinant HIV-1 and HIV-2 antigens were produced (3 Mab anti-gag protein, 2 anti-env1, and 5 anti-env2). In the immunoblotting assay all the anti-gag Mabs reacted with HIV capsid protein p24, whereas one of them reacted also with p55 protein and with 7 other polypeptides. Another anti-gag Mab cross-reacted with the antigen of subpopulation of human peripheral blood lymphocytes. The third one interacted with the antigen of both HIV-1 and HIV-2. All the 10 Mabs interacted with natural HIV antigens and can be used for identification and differentiation of HIV-1 and HIV-2.
Mice, Inbred BALB C, Hybridomas, HIV Antigens, Immunoblotting, Antibodies, Monoclonal, Fluorescent Antibody Technique, Gene Products, env, Gene Products, gag, Cross Reactions, Recombinant Proteins, Mice, HIV-2, HIV-1, Animals, Humans, Immunization
Mice, Inbred BALB C, Hybridomas, HIV Antigens, Immunoblotting, Antibodies, Monoclonal, Fluorescent Antibody Technique, Gene Products, env, Gene Products, gag, Cross Reactions, Recombinant Proteins, Mice, HIV-2, HIV-1, Animals, Humans, Immunization
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