[Concentration, purification, and isotopic labeling of sigma virus].

J, Bernard; A M, Petitjean

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Article . 1975

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[Concentration, purification, and isotopic labeling of sigma virus].

descriptionPublicationkeyboard_double_arrow_right Article 30 Oct 1975 French Journal:Comptes rendus hebdomadaires des seances de l'Academie des sciences. Serie D: Sciences naturelles, volume 280

Authors: J, Bernard; A M, Petitjean;

pmid: 807415

[Concentration, purification, and isotopic labeling of sigma virus].

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Abstract

Sigma virus has been concentrated by means of polyethylene-glycol and diafiltration; the latter method is more efficient in preserving infectivity. When purified on sucrose gradient, sigma banded in a sharp peak without any polydispersion. We are now able to label sigma genome with 3H-uridine in Drosophila cell cultures.

Keywords

Virus Cultivation, Isotope Labeling, Centrifugation, Density Gradient, Chemical Precipitation, RNA Viruses, Ultrafiltration, Drosophila, Polyethylenes, Uridine

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