Sensitivity of the platelet immunofluorescence test (PIFT) and the MAIPA assay for the detection of platelet-reactive alloantibodies: a report on two U.K. National Platelet Workshop exercises.

Name: Sensitivity of the platelet immunofluorescence test (PIFT) and the MAIPA assay for the detection of platelet-reactive alloantibodies: a report on two U.K. National Platelet Workshop exercises.
Keywords: Blood Platelets, Immunoassay, Isoantigens, Antibodies, Monoclonal, Fluorescent Antibody Technique, Reproducibility of Results, Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Sensitivity and Specificity, United Kingdom

D L, Allen; J, Chapman; P K, Phillips; W H, Ouwehand

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Transfusion Medicinearrow_drop_down

Transfusion Medicine

Article . 1994

Data sources: Europe PubMed Central

Sensitivity of the platelet immunofluorescence test (PIFT) and the MAIPA assay for the detection of platelet-reactive alloantibodies: a report on two U.K. National Platelet Workshop exercises.

descriptionPublicationkeyboard_double_arrow_right Article 22 Nov 1994 English Journal:Transfusion medicine (Oxford, England), volume 4 (issn: 0958-7578,

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Authors: D L, Allen; J, Chapman; P K, Phillips; W H, Ouwehand;

pmid: 7921052

Sensitivity of the platelet immunofluorescence test (PIFT) and the MAIPA assay for the detection of platelet-reactive alloantibodies: a report on two U.K. National Platelet Workshop exercises.

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Abstract

This report presents the results of two National Workshop exercises which were designed to evaluate interlaboratory and interassay variation in sensitivity of techniques used for the detection of platelet-reactive alloantibodies. Most workshop participants used the platelet immunofluorescence test. Sensitivity of this assay was improved when fluorescence was measured by flow cytometer rather than by microscope. The MAIPA assay was found to be highly sensitive but required considerable technical expertise, and the choice of antiglycoprotein IIb/IIIa (CD61/41) monoclonal antibody was found to have a significant effect on its ability to detect anti-HPA-1a.

Keywords

Blood Platelets, Immunoassay, Isoantigens, Antibodies, Monoclonal, Fluorescent Antibody Technique, Reproducibility of Results, Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Sensitivity and Specificity, United Kingdom, Antigen-Antibody Reactions, Antibody Specificity, Isoantibodies, Predictive Value of Tests, Humans

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