Synthetic nitrate saccharose (NS) nutrient media are offered to be used for Klebsiella pneumoniae. NS medium of the following composition is suggested for studies of substrates moderately contaminated with microorganisms: 20 g agar-agar, 1 g potassium nitrate, 2 g disubstituted potassium phosphate, monopotassium phosphate, 20 g saccharose, and 1 1 distilled water. NSK medium is offered for the isolation of Klebsiella from material abundantly contaminated with various bacteria; this medium includes, in addition to the said recipe, 10 ng/1 carbenicillin. Experiments and analysis of material from the patients demonstrated high sensitivity and inhibitory properties of NS media.