Liquid culture of Diplodia maydis.

Diplodia maydis, a Zea mays L. stalk rot causing fungus, was grown in Czapek-Dox broth and modifications of Fries liquid media using combinations of 1% cellulose, 1% sucrose and ammonium or nitrate-nitrogen. Conditions are defined to yield consistently maximum mycelial dry weight in two days in contrast to the usually reported five-day incubation period.

Keywords

Quaternary Ammonium Compounds, Sucrose, Glucose, Nitrates, Mitosporic Fungi, Cellulose, Zea mays, Culture Media, Plant Diseases

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