
Using UV-spectroscopy and circular dichroism (CD) phi KZ bacteriophage and its DNA were investigated. From the value of optical densities in the 320--400 nm range the size of the phi KZ bacteriophage's head was determined; the diameter of phi KZ bacteriophage head was found to be equal to 1300 +/- 100 A. phi KZ bacteriophage DNA has block structure and the GC-pair content is equal to 43.8 +/- 0.3%. phi KZ bacteriophage CD spectrum has an unusual profile (in comparison with known bacteriophage CD spectra); this spectrum is similar to the CD spectra of DNA in polyethylene glycole solution. To our knowledge CD spectra of such type were not obtained for other bacteriophages.
Chemistry, Cytosine, Hot Temperature, Chemical Phenomena, Guanosine, Circular Dichroism, DNA, Viral, Bacteriophages, Spectrophotometry, Ultraviolet, Nucleic Acid Denaturation
Chemistry, Cytosine, Hot Temperature, Chemical Phenomena, Guanosine, Circular Dichroism, DNA, Viral, Bacteriophages, Spectrophotometry, Ultraviolet, Nucleic Acid Denaturation
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