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Journal of microbiology epidemiology immunobiology

Article . 1983

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[Effectiveness of erythrocytic diagnostic reagents in indicating Shigellae].

descriptionPublicationkeyboard_double_arrow_right Article 17 Mar 1983 Russian Journal:Zhurnal mikrobiologii, epidemiologii i immunobiologii (issn: 0372-9311,

Authors: M Iu, Suslova; V A, Shamardin;

pmid: 7158163

[Effectiveness of erythrocytic diagnostic reagents in indicating Shigellae].

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Abstract

The possibility of the preparation of dysentery antibody diagnostic reagents on the basis of adsorbed sera is shown. Such diagnostic reagents permit to determine 200000-600000 microbial bodies or 0.0008-0.0016 microgram of the antigen per ml. The use of antigenic erythrocytic diagnostic reagents for the indication of shigellae is expedient in the second variant of the passive hemagglutination inhibition test in the following modification: immune sera should be diluted not 2-fold, but only 1.5-fold; the material to be tested for the presence of shigellae should be added to these dilutions in a double volume.

Keywords

Male, Antigens, Bacterial, Erythrocytes, Sheep, Hemagglutination Tests, Hemagglutination Inhibition Tests, Antibodies, Bacterial, Animals, Adsorption, Shigella

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