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Determination of fluorescence polarization of membrane probes in intact erythrocytes. Possible scattering artifacts.

Authors: Kutchai, Howard; Huxley, Virginia H.; Chandler, Laura H.;

Determination of fluorescence polarization of membrane probes in intact erythrocytes. Possible scattering artifacts.

Abstract

The anisotropy of the fluorescence of diphenylhexatriene has been reported to be less in the membranes of intact erythrocytes than in erythrocyte ghost membranes or in membranes prepared from erythrocyte lipids. Evidence is presented that this may be an artifact due to the intense light scattering by the intact erythrocytes.

Keywords

Membrane Lipids, Erythrocytes, Light, Erythrocyte Membrane, Humans, Scattering, Radiation, Cell Count, Fluorescence Polarization, Diphenylhexatriene

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    influence
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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
21
Average
Top 10%
Top 10%